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AlphaLISA SureFire Ultra Human & Mouse Phospho-Rb (Ser807/811) Detection Kit, Control Lysate

Extra control lysate for the AlphaLISA™ SureFire® Ultra™ phospho-Rb (Ser807/811) assay. This control is already provided in the AlphaLISA SureFire Ultra phospho-Rb assay kit but can also be ordered separately.

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MARCH10

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Feature Specification
Application Cell Signaling

Extra control lysate for the AlphaLISA™ SureFire® Ultra™ phospho-Rb (Ser807/811) assay. This control is already provided in the AlphaLISA SureFire Ultra phospho-Rb assay kit but can also be ordered separately.

Click to copy promo code to clipboard.
img-icon-save-10-black.svg

March lab savings - buy on Revvity.com or Revvity punchout to SAVE 10% with promo code!

MARCH10

Sale ends 3/24. Terms and conditions apply.

Product Variant
Unit Size: 1 vial
Part #:
ALSU-PRB-A-L
List Price
USD 151.56
Your online price:
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).

Overview

This control lysate is intended for use with the AlphaLISA SureFire Ultra Human and Mouse Phospho-Rb (Thr821) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, Jurkat cells were cultured in 10% FBS containing media. Cells were harvested andwashed with HBSS + 0.1% BSA, adjusted to 4 x106 cells/mL and then treated with 1.25 nM Pervanadate for 30 minutes. Cells were then lysed with the addition of 5X Lysis Buffer.

Specifications

Application
Cell Signaling
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra
Detection Modality
Alpha
Lysis Buffer Compatibility
Lysis Buffer
Molecular Modification
Phosphorylation
Product Group
Control Lysate
Shipping Conditions
Shipped in Blue Ice
Target Class
Phosphoproteins
Target Species
Human
Mouse
Technology
Alpha
Therapeutic Area
Oncology
Unit Size
1 vial

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Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

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