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AlphaLISA Surefire Ultra Human and Mouse Phospho-PDGF Receptor β (Tyr751) Detection Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra p-PDGF Receptor β assay is a sandwich immunoassay for quantitative detection of phosphorylated PDGF Receptor β in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-PPDGF-A500
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Part Number: ALSU-PPDGF-A10K
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Part Number: ALSU-PPDGF-A50K
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Part Number: ALSU-PPDGF-A-HV
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  • The AlphaLISA SureFire Ultra p-PDGF Receptor β (Tyr751) assay is a sandwich immunoassay for quantitative detection of phosphorylated PDGF Receptor β in cellular lysates using Alpha Technology.

    The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume. The 500 point kit contains reagents to run 500 wells in 384-well format, using a 20 μL reaction volume. The 10,000 point kit contains reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume. The 50,000 point kit contains reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.

    In the AlphaLISA SureFire Ultra assay, Donor Beads are coated with streptavidin to capture one of the, which is biotinylated. Acceptor Beads are coated with a proprietary CaptSureTM agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.

    AlphaLISA SureFire Ultra kits are compatible with:

    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies

    Alpha SureFire kits can be used for:

    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Application
    Protein Analysis & Detection
    Assay Points
    500
    Assay Target Class
    Protein
    Assay Technology
    AlphaLISA SureFire Ultra
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha

Resources

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Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra
surefire, neutralizing antibody, neutralizing antibodies, biotherapeutics, biotx