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AlphaLISA Surefire Ultra Total PLK1 Detection Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra Human and Mouse Total PLK1 assay is a sandwich immunoassay for quantitative detection of total PLK1 in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-TPLK1-A500
Unit Size: 500 Assay Points
Part Number: ALSU-TPLK1-A10K
Unit Size: 10,000 Assay Points
Part Number: ALSU-TPLK1-A50K
Unit Size: 50,000 Assay Points
Part Number: ALSU-TPLK1-A-HV
Unit Size: 100 Assay Points
  • Polo-like kinase 1 (PLK1) is a Ser/Thr kinase essential for cell division (mitosis) and as such, a key regulator of the G2/M checkpoint of the cell cycle. PLK1 is activated by phosphorylation of Thr210 by AurA kinase and active PLK1 phosphorylates CDC25. Overexpression of PLK1 is associated with many types of cancers, including lung, breast, liver, and osteosarcoma.

    The AlphaLISA SureFire Ultra Human and Mouse Total PLK1 is a sandwich immunoassay for the quantitative detection of total PLK1 in cellular lysates, using Alpha Technology.

    Formats
    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
    AlphaLISA SureFire Ultra kits are compatible with
    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies
    Alpha SureFire kits can be used for
    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Application
    Protein Analysis & Detection
    Protein Detection
    Assay Points
    10000
    Assay Target Class
    Phospho-protein
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Shipping Conditions
    Shipped in Blue Ice
    Target Species
    Human
    Mouse
    Therapeutic Area
    Neuroscience
    Oncology
    Unit Size
    10,000 Assay Points

Resources

1-2 of 2 Resources
Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

Application Note Icon Image
Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra

Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...