The AlphaLISA™ SureFire® Ultra™ total PLC G1 assay is a sandwich immunoassay for quantitative detection of Total (phosphorylated and non-phosphorylated) PLC G1in cellular lysates using Alpha Technology. This assay is intended to be a normalization phosphorylation studies.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Formats:
In the AlphaLISA SureFire Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.
AlphaLISA SureFire Ultra kits are compatible with:
Alpha SureFire kits can be used for:
Application |
Cell Signaling
|
---|---|
Assay Target |
PLC G1
|
Assay Target Class |
Phosphoproteins
|
Assay Technology |
Alpha
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Method |
Alpha
|
Molecular Modification |
Total
|
Product Group |
Kit
|
Sample Volume |
10 µL
|
Shipping Conditions |
Shipped in Blue Ice
|
Therapeutic Area |
Oncology
|
Unit Size |
500 Assay Points
|
This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...
Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...
Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
Are you looking for technical documents related to the product? We have categorized them in dedicated sections below. Explore now.
We are here to answer your questions.