PTEN-induced kinase 1 (PINK1) is a mitochondrial serine/threonine-protein kinase, closely involved in mitochondrial quality control by identifying damaged mitochondria and targeting specific mitochondria for degradation. In damaged depolarized mitochondria, PINK1 accumulates on the mitochondrial outer membrane. Upon activation, PINK1 activates Parkin which will ubiquitinate many targets at the Mitochondrial Outer Membrane (MOM). One of these targets is TOM20. Dysregulated PINK1 is associated with Alzheimer’s disease, Parkinson’s disease, Huntington’s disease, ALS, liver disorders, and kidney disorders.

The AlphaLISA SureFire Human Total PINK1 Detection Kit is a sandwich immunoassay for the quantitative detection of total PINK1 in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening