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AlphaLISA Surefire Ultra Total PI3 Kinase p85 Detection Kit, Control Lysate
Extra control lysate for the AlphaLISA® SureFire® Ultra™ Total PI3 Kinase p85 assay. This control is already provided in the AlphaLISA SureFire Ultra Total PI3 Kinase p85 assay kit, but can also be ordered separately.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product information
Overview
This control lysate is intended for use with the AlphaLISA SureFire Ultra Total PI3 Kinase p85 Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate NIH/3T3 cells were cultured to 80-90% confluence in T175 flasks in 10% FBS containing medium, medium removed and then treated with 50 ng/mL PDGF-BB for 5 minutes and lysed in 5 mL of 1X SureFire Ultra Lysis buffer.
Specifications
| Application |
Protein Detection
|
|---|---|
| Assay Technology |
Alpha
|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Method |
Alpha
|
| Experimental Type |
In vitro
|
| Unit Size |
1 vial
|
Video gallery
Resources
This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...
Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...
SDS, COAs, Manuals and more
Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.
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