Pyruvate dehydrogenase E1 subunit alpha 1 (PDHA1) is an enzyme involved in the pyruvate dehydrogenase complex (PDC). PDC is a multi-enzyme complex of the mitochondria that provides a link between glycolysis and the Krebs cycle and plays an essential role in producing acetyl-CoA from glucose leading to ATP or energy production. The PDHA1 enzyme is regulated by phosphorylation and dephosphorylation. Diseases associated with PDHA1 include pyruvate dehydrogenase E1-alpha deficiency, X-linked Leigh syndrome, and numerous solid tumor cancers.

The AlphaLISA SureFire Ultra Human and Mouse Phospho-PDHA1 (Ser293) is a sandwich immunoassay for the quantitative detection of phospho-PDHA1 (Ser293) in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• Screening