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AlphaLISA Surefire Ultra Total p27 KIP1 Detection Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra Human and Mouse Total p27 KIP1 assay is a sandwich immunoassay for quantitative detection of total p27 KIP1 in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-TP27K-A500
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Part Number: ALSU-TP27K-A10K
Unit Size:
Part Number: ALSU-TP27K-A50K
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Part Number: ALSU-TP27K-A-HV
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  • Cyclin-dependent kinase inhibitor 1B (p27 KIP1) is an enzyme inhibitor that is encoded by the CDKN1B gene. The p27 protein inhibits the activity of CDK2–cyclin E and CDK4–cyclin D complexes at the G1-phase and functions as a CDK-inhibitor that halts the S-phase transition. Mitogenic signals that decrease p27 expression allow CDK/cyclin activation and cell cycle progression. This protein participates in numerous signal transduction pathways and mediates tumor suppressor or oncogenic roles in cancers such as breast, colon, prostate, lung, esophageal, gastric, and ovarian.

    The AlphaLISA SureFire Human and Mouse Total p27 KIP1 Detection Kit is a sandwich immunoassay for the quantitative detection of total p27 KIP1 in cellular lysates, using Alpha Technology.

    Formats
    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
    AlphaLISA SureFire Ultra kits are compatible with
    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies
    Alpha SureFire kits can be used for
    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Application
    Protein Analysis & Detection
    Protein Detection
    Assay Target Class
    Phospho-protein
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Target Species
    Human
    Mouse
    Therapeutic Area
    Oncology