Extra control lysate for the AlphaLISA SureFire Ultra phospho-NFκB (Ser536) assay. These controls are already provided in the AlphaLISA SureFire Ultra phospho-NFκB assay kit, but can also be ordered separately.
Feature | Specification |
---|---|
Application | Cell Signaling |
Extra control lysate for the AlphaLISA SureFire Ultra phospho-NFκB (Ser536) assay. These controls are already provided in the AlphaLISA SureFire Ultra phospho-NFκB assay kit, but can also be ordered separately.
This control lysate is intended for use with the AlphaLISA SureFire Ultra phospho-NFκB p65 (Ser536) assay kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. This control lysate was prepared from HeLa cells, cultured to confluence in T175 flasks in 10% FBS containing medium for 3 days, then treated with 50 ng/mL TNFα and 20 ng/mL Calyculin A for 10 minutes and lysed in 10mL of 1X SureFire Ultra Lysis buffer.
Application |
Cell Signaling
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Modality |
Alpha
|
Lysis Buffer Compatibility |
Lysis Buffer
|
Molecular Modification |
Phosphorylation
|
Product Group |
Control Lysate
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
NFκB
|
Target Class |
Phosphoproteins
|
Target Species |
Human
Mouse
|
Technology |
Alpha
|
Therapeutic Area |
Metabolic
|
Unit Size |
1 Vial
|
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