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AlphaLISA Surefire Ultra Human Phospho-STAT5 (Tyr694/699) Detection Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra p-STAT5 (Tyr694/699) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-STAT5 (phosphorylated on Tyr694/699) in cellular lysates using Alpha Technology. This kit replaces ALSU-PST5-A-HV. ALSU-PST5-A-HV is no longer available as the antibody(ies) have been discontinued by the supplier. A new antibody pair has been selected and validated to make this new version, with a similar or slightly improved performance over the initial version. Comparative data are included in the manuals of the new kits.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: ALSU-PST5-B500
Unit Size: 500 Assay Points
List price: USD 2,346.00
Your price:
USD 2,346.00
USD 2,346.00 /each
Part number: ALSU-PST5-B10K
Unit Size: 10,000 Assay Points
List price: USD 14,270.00
Your price:
USD 0.00
USD 14,270.00 /each
Part number: ALSU-PST5-B50K
Unit Size: 50,000 Assay Points
List price: USD 44,270.00
Your price:
USD 0.00
USD 44,270.00 /each
Part number: ALSU-PST5-B-HV
Unit Size: 100 Assay Points
List price: USD 694.44
Your price:
USD 0.00
USD 694.44 /each

Overview

The AlphaLISA®SureFire®Ultra p-STAT5 (Tyr694/699) assay is a sandwich immunoassay for quantitative detection of phospho-STAT5 (phosphorylated on Tyr694/699) in cellular lysates using Alpha Technology.

In the AlphaLISA®SureFire®Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.

AlphaLISA® SureFire® Ultra™ kits are compatible with:

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Specifications

Application
Kinase
Protein Analysis & Detection
Assay Pathway
JAK/STAT
Assay Points
500
Assay Target Class
Phospho-protein
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra
Detection Method
Alpha
Experimental Type
In vitro
Format
Microplates
Quantity
1
Quantity in a Package Amount
1.0 Units
Second Messenger Release
Protein phosphorylation
Shipping Conditions
Shipped in Blue Ice
Unit Size
500 Assay Points

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Resources

1-5 of 5 Resources
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SDS, COAs, Manuals and more Illuminator

SDS, COAs, Manuals and more

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