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The AlphaLISA® SureFire® Ultra™ p-PI3 Kinase p85 (Tyr458) (Mouse) assay is a sandwich immunoassay for quantitative detection of phospho-PI3 Kinase p85 (phosphorylated on Tyr158) in cellular lysates using Alpha Technology.
In the AlphaLISA SureFire Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.
AlphaLISA SureFire Ultra kits are compatible with:
Alpha SureFire kits can be used for:
Protein Analysis & Detection
P13 Kinase p85
|Assay Target Class
AlphaLISA SureFire Ultra
10,000 Assay Points