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AlphaLISA Surefire Ultra Mouse Phospho-MLKL (Ser345) Detection Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra mouse Phospho-MLKL (Ser345) assay is a sandwich immunoassay for quantitative detection of Phospho-MLKL (Ser345) in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-PMLKL-A500
Unit Size: 500 Assay Points
Part Number: ALSU-PMLKL-A10K
Unit Size: 10,000 Assay Points
Part Number: ALSU-PMLKL-A50K
Unit Size: 50,000 Assay Points
Part Number: ALSU-PMLKL-A-HV
Unit Size: 100 Assay Points
  • Mixed lineage kinase domain-like protein is encoded by the MLKL gene in murine species. MLKL is a pseudokinase that plays a crucial role in TNF-induced necroptosis. MLKL is activated following phosphorylation by RIP3 kinase, which induces a conformational switch essential for necroptosis. The protein kinase domain of MLKL is catalytically inactive, but contains a pseudoactive site with an interaction between Lys-219 and Gln-343 residues. Upon phosphorylation by RIP3, MLKL undergoes an active conformation and homodimerization through its amino-terminal coiled-coil domain.

    The AlphaLISA® SureFire® Ultra™ mouse p-MLKL (Ser345) assay is a sandwich immunoassay for quantitative detection of Phospho-MLKL (Ser345) in cellular lysates using Alpha Technology.

    Formats:

    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

    AlphaLISA SureFire Ultra kits are compatible with:

    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies

    Alpha SureFire kits can be used for:

    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Application
    Protein Analysis & Detection
    Protein Detection
    Assay Target Class
    Phospho-protein
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Shipping Conditions
    Shipped in Blue Ice
    Therapeutic Area
    Inflammation
    Neuroinflammation
    Oncology
    Unit Size
    500 Assay Points

Resources

1-3 of 3 Resources
Brochure Icon Image
Brochure
Alpha SureFire Ultra no-wash immunoassay catalog

Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...

Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

Application Note Icon Image
Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra

Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...