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AlphaLISA SureFire Ultra Human Phospho-MLKL (Ser358) Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human Phospho-MLKL (Ser358) Detection Kit, Control Lysate
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Phospho-MLKL (Ser358) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-MLKL (Ser358) assay kit, but can also be ordered separately.
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Phospho-MLKL (Ser358) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-MLKL (Ser358) assay kit, but can also be ordered separately.
AlphaLISA SureFire Ultra Human Phospho-MLKL (Ser358) Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human Phospho-MLKL (Ser358) Detection Kit, Control Lysate
Product information
Overview
This control lysate is intended for use with the AlphaLISA SureFire Ultra Phospho-MLKL (Ser358) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, U937 cells were cultured in T175 flasks to 106 cells/mL in RPMI containing 10% FBS. Cells were treated with 20 μM Z-VAD for 30 minutes at 37°C, and then treated with 100 nM SM-164 and 20 ng/mL TNFα for 4 hours at 37°C. Total stimulus time 4.5 hours. Cells were then washed with HBSS and lysed in 5 mL 1X SureFire Ultra Lysis buffer.
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Phosphorylation
|
| Product Group |
Control Lysate
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
MLKL
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Unit Size |
1 vial
|
Video gallery
AlphaLISA SureFire Ultra Human Phospho-MLKL (Ser358) Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human Phospho-MLKL (Ser358) Detection Kit, Control Lysate
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