AlphaLISA SureFire Ultra Human Total LCK Detection Kit, Control Lysate

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AlphaLISA SureFire Ultra Human Total LCK Detection Kit, Control Lysate

SDS

Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Total LCK assay. This control is already provided in the AlphaLISA SureFire Ultra Total LCK assay kit, but can also be ordered separately.

Product information

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

SDS

Product information

Overview

This control lysate is intended for use with the AlphaLISA SureFire Ultra Total LCK Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. The lysate is prepared from Jurkat cells, cultured in a T175 flask to approximately 1 x 106 cells/mL in 10% FBS containing medium. Cells were resuspended in HBSS at 4 x 106 cells/mL and treated with 11 mM Peroxide plus 50 nM Calyculin A for 10 minutes. Cells were lysed with 5X SureFire Ultra Lysis Buffer at a final density of 3.2 x 106 cells/mL.

Specifications

Other Specifications

Application	Protein Detection
Assay Target	LCK
Assay Technology	Alpha
Automation Compatible	Yes
Brand	AlphaLISA SureFire Ultra
Detection Method	Alpha
Shipping Conditions	Shipped in Blue Ice
Unit Size	1 vial