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AlphaLISA SureFire Ultra Human & Mouse Total JAK2 Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human & Mouse Total JAK2 Detection Kit, Control Lysate
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Total JAK12 assay. This control is already provided in the AlphaLISA SureFire Ultra Total JAK12 assay kit, but can also be ordered separately.
| Feature | Specification |
|---|---|
| Application | Protein Quantification |
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Total JAK12 assay. This control is already provided in the AlphaLISA SureFire Ultra Total JAK12 assay kit, but can also be ordered separately.
AlphaLISA SureFire Ultra Human & Mouse Total JAK2 Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human & Mouse Total JAK2 Detection Kit, Control Lysate
Product information
Overview
This control lysate is intended for use with the AlphaLISA SureFire Ultra Total JAK12 Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFireassay. The lysate is prepared from EL92.1.7 cells seeded at 500K cells/mL and incubated overnight in 10% FBS containing medium. Cells were harvested, washed in HBSS + 0.1% BSA, adjusted to 4 x 106 cells/mL and treated with 0.5 mM Pervanadate for 15 minutes. Following treatment, cells were spun down and lysed with Lysis Buffer to a final concentration of 4 x 106 cells/mL.
Specifications
| Application |
Protein Quantification
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Total
|
| Product Group |
Control Lysate
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
JAK12
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
Mouse
|
| Technology |
Alpha
|
| Unit Size |
1 vial
|
Video gallery
AlphaLISA SureFire Ultra Human & Mouse Total JAK2 Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human & Mouse Total JAK2 Detection Kit, Control Lysate
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