Revvity Sites Globally
Select your location.
*e-commerce not available for this region.
AlphaLISA SureFire Ultra Mouse Phospho-IRF5 (Ser446) Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Mouse Phospho-IRF5 (Ser446) Detection Kit, Control Lysate
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human Phospho-IRF5 (Ser446) assay. This control is already provided in the AlphaLISA SureFire Ultra Human Phospho-IRF5 (Ser446) assay kit, but can also be ordered separately.
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human Phospho-IRF5 (Ser446) assay. This control is already provided in the AlphaLISA SureFire Ultra Human Phospho-IRF5 (Ser446) assay kit, but can also be ordered separately.
AlphaLISA SureFire Ultra Mouse Phospho-IRF5 (Ser446) Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Mouse Phospho-IRF5 (Ser446) Detection Kit, Control Lysate
Product information
Overview
This control lysate is intended for use with the AlphaLISA SureFire Ultra Human Phospho-IRF5 (Ser446) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, THP-1 cells were seeded at 300K/mL and incubated for 48 hours in T175 flasks in 10% FBS containing medium. Cells were harvested and resuspended at 8 x 106 cells/mL and then treated with 100 nM Calyculin A for 3 hours. Cells were then washed in HBSS + 0.1% BSA and lysed in Lysis Buffer at a final cell density of 8x106 cells/mL.
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Phosphorylation
|
| Product Group |
Control Lysate
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
IRF5
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Therapeutic Area |
Inflammation
Oncology
|
| Unit Size |
1 vial
|
Resources
Are you looking for resources, click on the resource type to explore further.
How can we help you?
We are here to answer your questions.
