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AlphaLISA Surefire Ultra Human Phospho-IRAK4 (Thr345/Ser346) Detection Kit, Control Lysate

Extra control lysate for the AlphaLISA® SureFire® Ultra Human Phospho-IRAK4 (Thr345/Ser346) assay. This control is already provided in the AlphaLISA SureFire Ultra Human Phospho-IRAK4 (Thr345/Ser346) assay kit, but can also be ordered separately.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part number: ALSU-PIRAK-A-L
List price: USD 142.66
Your price:
USD 142.66
USD 142.66 /each

Overview

This control lysate is intended for use with the AlphaLISA SureFire Ultra Human p-IRAK4 (Thr345/Ser346) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, Karpas299 cells were seeded at 200K/mL and grown for two days in 100 mL of 10% FBS containing medium. Cells were harvested and washed in HBSS + 0.1% BSA, adjusted to 4 X 106 cells/mL and then treated with 10 ng/mL IL-1β for 10 minutes. Cells were spun down at 1000 g for 5 mins and lysed in 10 mL of SureFire Ultra Lysis Buffer.

Specifications

Application
Protein Detection
Assay Technology
Alpha
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra
Detection Method
Alpha
Experimental Type
In vitro
Target Species
Human
Therapeutic Area
Autoimmunity
Immuno-oncology
Inflammation
Unit Size
1 vial

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