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AlphaLISA SureFire Ultra Human Phospho-IRAK4 (Thr345/Ser346) Detection Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra Human Phospho-IRAK4 (Thr345/Ser346) assay is a sandwich immunoassay for quantitative detection of phospho-IRAK4 (Thr345/Ser346) in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-PIRAK-A50K
Unit Size: 50,000 Assay Points
Part Number: ALSU-PIRAK-A-HV
Unit Size: 100 Assay Points
Part Number: ALSU-PIRAK-A10K
Unit Size: 10,000 Assay Points
Part Number: ALSU-PIRAK-A500
Unit Size: 500 Assay Points
  • IRAK4 (IL-1R-associated kinase 4) is an essential serine-threonine kinase in the signaling cascade associated with toll-like receptors (TLRs). Through NF-κB-mediated and mitogen-activated protein kinase (MAPK) activation as well as interferon response factors 3 and 7 (IRF3/7), IRAK4 is implicated in the generation of proinflammatory cytokines. IRAK4 is a key player in the IL-1/TLR signaling pathway because, unlike other IRAK proteins, it fully inhibits TLR receptor activation when it is suppressed. IRAK4 is an important drug therapy target for various cancers and inflammatory disorders including rheumatoid arthritis, inflammatory bowel disease, and other autoimmune diseases.

    The AlphaLISA SureFire Human Phospho-IRAK4 (Thr345/Ser346) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-IRAK4 (Thr345/Ser346) in cellular lysates, using Alpha Technology.

    Formats
    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
    AlphaLISA SureFire Ultra kits are compatible with
    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies
    Alpha SureFire kits can be used for
    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Automation Compatible
    Yes
    Application
    Protein Detection
    Assay Target
    IRAK4
    Assay Technology
    Alpha
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Target Species
    Human
    Therapeutic Area
    Autoimmunity
    Immuno-oncology
    Inflammation
    Brand
    AlphaLISA SureFire Ultra
    Unit Size
    50,000 Assay Points