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Extra control lysate for the AlphaLISA® SureFire® Ultra™ Total IKKβ assay. This control is already provided in the AlphaLISA SureFire Ultra Total IKKβ assay kit, but can also be ordered separately.
This control lysate is intended for use with the AlphaLISA SureFire Ultra Human Total IKKβ Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, THP1 cells were cultured in T175 flasks to 0.75 x 106 cells/mL in medium containing 10% FBS, then treated with 50 μg/mL LPS for 1 hour and lysed at 10 x 106 cells/mL in SureFire Ultra Lysis Buffer.
AlphaLISA SureFire Ultra