Extra control lysate for the AlphaLISA® SureFire® Ultra™ Phospho-IKKβ (Ser177/181) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-IKKβ (Ser177/181) assay kit, but can also be ordered separately.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
This control lysate is intended for use with the AlphaLISA SureFire Ultra Human Phospho-IKKβ (Ser177/181) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, THP1 cells were cultured in T175 flasks to 0.75 x 106 cells/mL in medium containing 10% FBS, then treated with 50 μg/mL LPS for 1 hour and lysed at 10 x 106 cells/mL in 1X SureFire Ultra Lysis Buffer.
Application |
Protein Detection
|
---|---|
Assay Technology |
Alpha
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Method |
Alpha
|
Experimental Type |
In vitro
|
Target Species |
Human
|
Therapeutic Area |
Autoimmunity
Immuno-oncology
Oncology
|
Unit Size |
1 vial
|
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