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AlphaLISA SureFire Ultra Human and Mouse Total Ikaros Detection Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra Human and Mouse Total Ikaros assay is a sandwich immunoassay for quantitative detection of total Ikaros in cellular lysates using Alpha Technology.

Part Number: ALSU-TIKARO-A500
Unit Size:
Part Number: ALSU-TIKARO-A10K
Unit Size:
Part Number: ALSU-TIKARO-A50K
Unit Size:
Part Number: ALSU-TIKARO-A-HV
Unit Size:
  • Ikaros, or IKZF1, is a transcription factor and a member of the Krüppel family, also known as the Ikaros family zinc finger protein family (IKZF). Ikaros displays crucial functions in the hematopoietic system and is a known regulator of immune cell development. Ikaros can also directly initiate or suppress tumor suppressors or oncogenes, consequently regulating the survival and proliferation of cancer cells. Mutations or deficiencies associated with Ikaros are linked to numerous cancers, including pediatric B-cell precursor acute lymphoblastic leukemia, multiple myeloma, acute lymphoblastic leukemia, mantle cell lymphoma, lung cancer, and ovarian cancer.

    The AlphaLISA SureFire Human and Mouse Total Ikaros Detection Kit is a sandwich immunoassay for the quantitative detection of total Ikaros in cellular lysates, using Alpha Technology.

    Formats:
    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
    AlphaLISA SureFire Ultra kits are compatible with:
    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies
    Alpha SureFire kits can be used for:
    • Cellular kinase assays
    • Receptor activation studies
    • Screening

    For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

  • Application
    Protein Analysis & Detection
    Protein Detection
    Assay Target Class
    Phospho-protein
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Target Species
    Human
    Mouse
    Therapeutic Area
    Oncology