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AlphaLISA SureFire Ultra Human Phospho-HSP27 (Ser15) Detection Kit, 100 Assay Points

The AlphaLISA® SureFire® Ultra Human Phospho-HSP27 (Ser15) assay is a sandwich immunoassay for quantitative detection of phopho-HSP27 (Ser15) in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-PHSP-A500
Unit Size:
Part Number: ALSU-PHSP-A10K
Unit Size:
Part Number: ALSU-PHSP-A50K
Unit Size:
Part Number: ALSU-PHSP-A-HV
Unit Size:
  • Heat shock protein 27 (Hsp27) is a protein that in humans is encoded by the HSPB1 gene. Hsp27 is a multidimensional protein which acts as a protein chaperone and an antioxidant and plays a role in the inhibition of apoptosis and actin cytoskeletal remodeling. In each of these capacities, HSP27 has been implicated in different disease states playing both protective and counter-protective roles. This protein has been indicated in renal injury, fibrosis, cancer, neurodegenerative disease, and cardiovascular disease.

    The AlphaLISA SureFire Human Phospho-HSP27 (Ser15) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-HSP27 (Ser15) in cellular lysates, using Alpha Technology.

    Formats
    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
    AlphaLISA SureFire Ultra kits are compatible with
    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies
    Alpha SureFire kits can be used for
    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Application
    Protein Analysis & Detection
    Protein Detection
    Assay Points
    100
    Assay Target Class
    Phospho-protein
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Target Species
    Human
    Therapeutic Area
    Cardiovascular
    Metabolic
    Neuroscience
    Oncology

Resources

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Application Note Icon Image
Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra
surefire, neutralizing antibody, neutralizing antibodies, biotherapeutics, biotx