Histone H3 is one of the five main histones involved in the structure of chromatin in eukaryotic cells. Histone H3 is the most extensively modified of the five histones and is phosphorylated during mitosis and meiosis. Aurora kinase B is directly responsible for mitotic Histone H3 Ser10 phosphorylation. Aurora kinase A is amplified in human cancers, with its overexpression in human tumors correlating with a poor prognosis and genomic instability. Histone H3 is implicated in glioblastomas, ovarian carcinomas, and hepatocellular carcinomas.

The AlphaLISA SureFire Ultra Human and Mouse Total Histone H3 assay is a sandwich immunoassay for the quantitative detection of total histone H3 in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening