Skip to main content

AlphaLISA Surefire Ultra Human Total FGFR3 Detection Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ total FGF Receptor 3 assay is a sandwich immunoassay for quantitative detection of FGF Receptor 3 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization phosphorylation studies.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-TFGFR3-A500
Unit Size: 500 Assay Points
Part Number: ALSU-TFGFR3-A10K
Unit Size: 10,000 Assay Points
Part Number: ALSU-TFGFR3-A50K
Unit Size: 50,000 Assay Points
Part Number: ALSU-TFGFR3-A-HV
Unit Size: 100 Assay Points
  • The AlphaLISA® SureFire® Ultra™ total FGF Receptor 3 assay is a sandwich immunoassay for quantitative detection of total FGF Receptor 3 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology.

    Formats:

    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

    In the AlphaLISA SureFire Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.

    AlphaLISA SureFire Ultra kits are compatible with:

    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies

    Alpha SureFire kits can be used for:

    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Application
    Protein Analysis & Detection
    Assay Points
    10000
    Assay Target Class
    Phospho-protein
    Protein
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Shipping Conditions
    Shipped in Blue Ice
    Therapeutic Area
    Oncology
    Unit Size
    10,000 Assay Points

Resources

1-2 of 2 Resources
Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

Application Note Icon Image
Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra

Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...