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AlphaLISA Surefire Ultra Human and Mouse Total FAK Detection Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra Human and Mouse Total FAK assay is a sandwich immunoassay for quantitative detection of total FAK in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-TFAK-A500
Unit Size:
Part Number: ALSU-TFAK-A10K
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Part Number: ALSU-TFAK-A50K
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Part Number: ALSU-TFAK-A-HV
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  • Focal adhesion kinase (FAK), also known as protein tyrosine kinase 2 (PTK2), is a non-receptor tyrosine kinase that can be activated by growth factor receptors, cytokine receptors, and GPCRs. FAK is a central regulator of ECM/integrin and cadherin signaling and plays a role in cell adhesion, cell junction, migration, survival, and mechanosensing. Increased FAK expression and phosphorylation is implicated in increased tumor cell adhesion, tumor cell motility and invasion, and with fibroblast migration on fibronectin in tissue fibrosis.

    The AlphaLISA SureFire Human and Mouse Total FAK Detection Kit is a sandwich immunoassay for the quantitative detection of total FAK in cellular lysates, using Alpha Technology.

    Formats
    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
    AlphaLISA SureFire Ultra kits are compatible with
    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies
    Alpha SureFire kits can be used for
    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Application
    Protein Analysis & Detection
    Protein Detection
    Assay Target Class
    Phospho-protein
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Target Species
    Human
    Mouse
    Therapeutic Area
    Oncology

Resources

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Application Note Icon Image
Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra
surefire, neutralizing antibody, neutralizing antibodies, biotherapeutics, biotx