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AlphaLISA Surefire Ultra Human, Mouse, and Rat Phospho-ERK1/2 (Thr202/Tyr204) Detection Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra p-ERK 1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK 1/2 (phosphorylated on Thr202/Tyr204 in ERK1, or Thr185/Tyr187 in ERK2) in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-PERK-A500
Unit Size:
Part Number: ALSU-PERK-A10K
Unit Size:
Part Number: ALSU-PERK-A50K
Unit Size:
Part Number: ALSU-PERK-A-HV
Unit Size:
  • Formats:

    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 µL reaction volume.

    In the AlphaLISA®SureFire®Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.

    AlphaLISA®SureFire®Ultra kits are compatible with:

    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies

    Alpha SureFire® kits can be used for:

    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Assay Pathway
    MAPK
    Assay Target Class
    Phospho-protein
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Format
    Microplates
    Quantity in a Package Amount
    50000.0 Units
    Therapeutic Area
    Cardiovascular
    Central Nervous System
    Neuroinflammation

Resources

1-2 of 2 Resources
Application Note Icon Image
Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra
surefire, neutralizing antibody, neutralizing antibodies, biotherapeutics, biotx
Application Note Icon Image
Application Note
Protein extraction using the Omni Bead Ruptor Elite with detection of phosphorylation events using AlphaLISA no-wash assays
protein extraction, immunoassays, homogenizer