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AlphaLISA Surefire Ultra Human Total ERα Detection Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra Human Total Estrogen Receptor α (ERα) assay is a sandwich immunoassay for quantitative detection of total ERα in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-TERA-A10K
Unit Size: 10,000 Assay Points
Part Number: ALSU-TERA-A500
Unit Size: 500 Assay Points
Part Number: ALSU-TERA-A50K
Unit Size: 50,000 Assay Points
Part Number: ALSU-TERA-A-HV
Unit Size: 100 Assay Points
  • Estrogen Receptors (ERs) are a group of proteins found inside cells and exist in two classes: ERα and ERβ. ERs are activated by estrogen and are able to translocate into the nucleus and bind to DNA to regulate the activity of different genes, but also have functions independent of DNA binding. ERs are expressed in >70% of all breast cancers with ERα being the main target in clinical approaches for controlling hormonally responsible breast cancer. ERα is associated with a variety of other cancers, including prostate, liver, and pancreatic ductal adenocarcinoma.

    The AlphaLISA SureFire Ultra Human Total ERα Detection Kit is a sandwich immunoassay for the quantitative detection of total ERα in cellular lysates, using Alpha Technology.

    Formats:
    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
    AlphaLISA SureFire Ultra kits are compatible with:
    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies
    Alpha SureFire kits can be used for:
    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Automation Compatible
    Yes
    Application
    Protein Analysis & Detection
    Protein Detection
    Assay Points
    10000
    Assay Target
    ERα
    Assay Target Class
    Phospho-protein
    Assay Technology
    Alpha
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Target Species
    Human
    Therapeutic Area
    Oncology
    Brand
    AlphaLISA SureFire Ultra
    Unit Size
    10,000 Assay Points

Resources

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Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra
SureFire, neutralizing antibody, neutralizing antibodies, biotherapeutics, biotx