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The positive control lysate is prepared from C2C12 cells, cultured to confluence in T175 flasks in 10% FBS containing medium for 3 days, then treated with thapsigargin (1 µM) for 30min and lysed in 4 mL of 1X SureFire Ultra Lysis buffer.
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Phosphorylation
|
| Product Group |
Control Lysate
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
eIF2α
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
Mouse
|
| Technology |
Alpha
|
| Unit Size |
1 vial
|
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