Skip to main content

AlphaLISA Surefire Ultra Total EGFR L858R Detection Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra Human Total EGF Receptor L858R assay is a sandwich immunoassay for quantitative detection of total EGF Receptor L858R in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-TEGFR-B500
Unit Size: 500 Assay Points
Part Number: ALSU-TEGFR-B10K
Unit Size: 10,000 Assay Points
Part Number: ALSU-TEGFR-B50K
Unit Size: 50,000 Assay Points
Part Number: ALSU-TEGFR-B-HV
Unit Size: 100 Assay Points
  • Epidermal growth factor receptor (EGFR, EGF Receptor, Erb-1, or HER1) is a transmembrane protein and receptor for members of the epidermal growth factor (EGF) family. Binding of EGFR ligands drives receptor homodimerization or hetero-dimerization, leading to the activation of the EGFR tyrosine kinase domain and specific tyrosine residues. The signals transmitted from the EGF receptor to the nucleus lead to the regulation of various biological functions, such as cell proliferation, differentiation, survival, adhesion, migration, and angiogenesis. Mutations in EGFR, including mutation L858R in exon 21, result in EGFR overexpression and are associated with cancer.

    The AlphaLISA SureFire Human Total EGF Receptor L858R Detection Kit is a sandwich immunoassay for the quantitative detection of total EGF Receptor L858R in cellular lysates, using Alpha Technology.

    Formats
    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
    AlphaLISA SureFire Ultra kits are compatible with
    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies
    Alpha SureFire kits can be used for
    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Application
    Protein Analysis & Detection
    Protein Detection
    Assay Target
    EGF Receptor
    Assay Target Class
    Phospho-protein
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Target Species
    Human
    Therapeutic Area
    Immuno-oncology
    Oncology
    Unit Size
    500 Assay Points

Resources

1-1 of 1 Resources
Application Note Icon Image
Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra
SureFire, neutralizing antibody, neutralizing antibodies, biotherapeutics, biotx