Skip to main content

AlphaLISA Surefire Ultra Total EGFR L858R Detection Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra Human Total EGF Receptor L858R assay is a sandwich immunoassay for quantitative detection of total EGF Receptor L858R in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Part number: ALSU-TEGFR-B500
Unit Size: 500 Assay Points
List price: USD 2,346.00
Your price:
USD 2,346.00
USD 2,346.00 /each
Part number: ALSU-TEGFR-B10K
Unit Size: 10,000 Assay Points
List price: USD 14,270.00
Your price:
USD 0.00
USD 14,270.00 /each
Part number: ALSU-TEGFR-B50K
Unit Size: 50,000 Assay Points
List price: USD 46,060.00
Your price:
USD 0.00
USD 46,060.00 /each
Part number: ALSU-TEGFR-B-HV
Unit Size: 100 Assay Points
List price: USD 694.44
Your price:
USD 0.00
USD 694.44 /each

Overview

Epidermal growth factor receptor (EGFR, EGF Receptor, Erb-1, or HER1) is a transmembrane protein and receptor for members of the epidermal growth factor (EGF) family. Binding of EGFR ligands drives receptor homodimerization or hetero-dimerization, leading to the activation of the EGFR tyrosine kinase domain and specific tyrosine residues. The signals transmitted from the EGF receptor to the nucleus lead to the regulation of various biological functions, such as cell proliferation, differentiation, survival, adhesion, migration, and angiogenesis. Mutations in EGFR, including mutation L858R in exon 21, result in EGFR overexpression and are associated with cancer.

The AlphaLISA SureFire Human Total EGF Receptor L858R Detection Kit is a sandwich immunoassay for the quantitative detection of total EGF Receptor L858R in cellular lysates, using Alpha Technology.

Formats
  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
AlphaLISA SureFire Ultra kits are compatible with
  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies
Alpha SureFire kits can be used for
  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Specifications

Application
Protein Analysis & Detection
Protein Detection
Assay Target Class
Phospho-protein
Assay Technology
Alpha
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra
Detection Method
Alpha
Experimental Type
In vitro
Target Species
Human
Therapeutic Area
Immuno-oncology
Oncology
Unit Size
500 Assay Points

Video gallery

Resources

1-3 of 3 Resources
Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

Brochure Icon Image
Brochure
Alpha SureFire Ultra no-wash immunoassay catalog

Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...

Application Note Icon Image
Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra

Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...

SDS, COAs, Manuals and more Illuminator

SDS, COAs, Manuals and more

Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.

Scroll Icon