Parkinson disease protein 7, also known as protein deglycase DJ-1 or simply DJ-1, is a multifunctional enzyme that is highly conserved and has been identified in both prokaryotes and eukaryotes. This protein is involved in a variety of cellular processes, such as anti-oxidation, proteostatis, and cell proliferation. DJ-1 is a regulator involved in many cellular processes (e.g., epigenetic regulations) and a promising therapeutic target for a variety of diseases due to its complex enzymatic and non-enzymatic activities and transcriptional coactivator function.

The AlphaLISA SureFire Ultra Human and Mouse Total DJ1 Detection Kit is a sandwich immunoassay for the quantitative detection of total DJ1 in cellular lysates, using Alpha Technology.

 Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• Screening