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AlphaLISA Surefire Ultra Total Cyclin E1 Detection Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra Human Total Cyclin E1 assay is a sandwich immunoassay for quantitative detection of total cyclin E1 in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-TCYCE1-A500
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Part Number: ALSU-TCYCE1-A10K
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Part Number: ALSU-TCYCE1-A50K
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Part Number: ALSU-TCYCE1-A-HV
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  • Cyclin E1 (CCNE1) is a member of the highly conserved cyclin family, whose members have various roles in the cell cycle. CCNE1 forms a complex with cyclin-dependent kinase 2 (CDK2) and acts as a regulatory subunit. CDK2 is required for cell cycle G1/S transition. Overexpression of CCNE1 has been observed in many tumors, such as breast, ovarian, endometrial, and bladder cancer.

    The AlphaLISA SureFire Human Total Cyclin E1 Detection Kit is a sandwich immunoassay for the quantitative detection of total Cyclin E1 in cellular lysates, using Alpha Technology.

    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
    AlphaLISA SureFire Ultra kits are compatible with
    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies
    Alpha SureFire kits can be used for
    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Application
    Protein Analysis & Detection
    Protein Detection
    Assay Target Class
    Assay Technology
    Automation Compatible
    AlphaLISA SureFire Ultra
    Detection Method
    Experimental Type
    In vitro
    Target Species
    Therapeutic Area


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Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra
surefire, neutralizing antibody, neutralizing antibodies, biotherapeutics, biotx