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AlphaLISA SureFire Ultra Human Phospho-Cyclin B1 (Ser126) Detection Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra Human Phospho-Cyclin B1 (Ser126) assay is a sandwich immunoassay for quantitative detection of phospho-Cyclin B1 (Ser126) in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-PCYCB1-A500
Unit Size: 500 Assay Points
Part Number: ALSU-PCYCB1-A-HV
Unit Size: 100 Assay Points
Part Number: ALSU-PCYCB1-A10K
Unit Size: 10,000 Assay Points
Part Number: ALSU-PCYCB1-A50K
Unit Size: 50,000 Assay Points
  • G2/mitotic-specific cyclin-B1 (Cyclin B1) is a key component in the control of cell cycle progression from G2 to M phase and has been implicated in the tumorigenesis and the development of malignancy. Under DNA damage conditions, Cyclin B1 becomes upregulated, presumably already in the DNA replication phase (S-phase). This upregulation is controlled by SOG1, which in turn is activated by the checkpoint kinases ATM and ATR, which sense DNA damage. Cyclin B1/CDK1 regulate mitochondrial bioenergetics for cell cycle progression and tumor response to therapy. Cyclin B1 is associated with breast, colorectal, ovarian, lung, and pancreatic cancer.

    The AlphaLISA SureFire Human Phospho-Cyclin B1 (Ser126) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-Cyclin B1 (Ser126) in cellular lysates, using Alpha Technology.

    Formats
    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
    AlphaLISA SureFire Ultra kits are compatible with
    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies
    Alpha SureFire kits can be used for
    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Application
    Protein Analysis & Detection
    Protein Detection
    Assay Target
    Cyclin B1
    Assay Target Class
    Phospho-protein
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Target Species
    Human
    Therapeutic Area
    Oncology
    Unit Size
    500 Assay Points

Resources

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Application Note Icon Image
Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra
SureFire, neutralizing antibody, neutralizing antibodies, biotherapeutics, biotx