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AlphaLISA SureFire Ultra Human Phospho-Cyclin B1 (Ser126) Detection Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra Human Phospho-Cyclin B1 (Ser126) assay is a sandwich immunoassay for quantitative detection of phospho-Cyclin B1 (Ser126) in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Part number: ALSU-PCYCB1-A500
Unit Size: 500 Assay Points
List price: USD 2,346.00
Your price:
USD 2,346.00
USD 2,346.00 /each
Part number: ALSU-PCYCB1-A10K
Unit Size: 10,000 Assay Points
List price: USD 14,270.00
Your price:
USD 0.00
USD 14,270.00 /each
Part number: ALSU-PCYCB1-A50K
Unit Size: 50,000 Assay Points
List price: USD 46,060.00
Your price:
USD 0.00
USD 46,060.00 /each
Part number: ALSU-PCYCB1-A-HV
Unit Size: 100 Assay Points
List price: USD 694.44
Your price:
USD 0.00
USD 694.44 /each

Overview

G2/mitotic-specific cyclin-B1 (Cyclin B1) is a key component in the control of cell cycle progression from G2 to M phase and has been implicated in the tumorigenesis and the development of malignancy. Under DNA damage conditions, Cyclin B1 becomes upregulated, presumably already in the DNA replication phase (S-phase). This upregulation is controlled by SOG1, which in turn is activated by the checkpoint kinases ATM and ATR, which sense DNA damage. Cyclin B1/CDK1 regulate mitochondrial bioenergetics for cell cycle progression and tumor response to therapy. Cyclin B1 is associated with breast, colorectal, ovarian, lung, and pancreatic cancer.

The AlphaLISA SureFire Human Phospho-Cyclin B1 (Ser126) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-Cyclin B1 (Ser126) in cellular lysates, using Alpha Technology.

Formats
  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
AlphaLISA SureFire Ultra kits are compatible with
  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies
Alpha SureFire kits can be used for
  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Specifications

Application
Protein Analysis & Detection
Protein Detection
Assay Target Class
Phospho-protein
Assay Technology
Alpha
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra
Detection Method
Alpha
Experimental Type
In vitro
Target Species
Human
Therapeutic Area
Oncology
Unit Size
500 Assay Points

Video gallery

Resources

1-4 of 4 Resources
Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

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Brochure
Alpha SureFire Ultra no-wash immunoassay catalog

Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...

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Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra

Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...

Technical Note
Detecting changes in phosphorylation events during the cell cycle

Lamin A/C, a crucial nuclear protein, undergoes phosphorylation at mitotic sites, controlled by the CDK1-Cyclin B1 complex...

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SDS, COAs, Manuals and more

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