AlphaLISA SureFire Ultra Human & Mouse Total Cyclin A2 Detection Kit, 500 Assay Points

Cyclin A2 belongs to the highly conserved cyclin family, whose members function as regulators of the cell cycle. This protein binds and activates cyclin-dependent kinase 2 and thus promotes transition through G1/S and G2/M. Autophagy plays a role in cyclin A2 degradation to prevent its accumulation at the end of mitosis. Cyclin A2 is usually linked to cell proliferation and as such is often found expressed at a high level in human cancers.

The AlphaLISA SureFire Ultra Human and Mouse Total Cyclin A2 Detection Kit is a sandwich immunoassay for the quantitative detection of total Cyclin A2 in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening