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AlphaLISA SureFire Ultra Human Total CHK-2 Detection Kit, Control Lysate
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AlphaLISA SureFire Ultra Human Total CHK-2 Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Control Lysate
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Total CHK2 assay. This control is already provided in the AlphaLISA SureFire Ultra Total CHK2 assay kit, but can also be ordered separately.
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
| Protocol Time | 2h at RT |
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Total CHK2 assay. This control is already provided in the AlphaLISA SureFire Ultra Total CHK2 assay kit, but can also be ordered separately.
Product variant
Unit Size: 1 vial
Part #:
ALSU-TCHK2-A-L
List price
USD 159.00
Your price:
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaLISA SureFire Ultra Human Total CHK-2 Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Control Lysate
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Product information
Overview
This control lysate is intended for use with the AlphaLISA SureFire Ultra Total CHK2 Assay Kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, HEK293 cells were cultured to confluence in T175 flasks in 10% FBS containing medium, then treated with 5 μM Doxorubicin HCl for 30 minutes and lysed in 4 mL of 1X SureFire Ultra Lysis buffer.
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Cellular or Signaling Pathway |
Cell cycle regulation
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Total
|
| Product Group |
Control Lysate
|
| Protocol Time |
2h at RT
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
CHK-2
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Therapeutic Area |
Oncology
|
| Unit Size |
1 vial
|
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AlphaLISA SureFire Ultra Human Total CHK-2 Detection Kit, Control Lysate
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Application Note
Characterizing chemokine receptor inhibitors with AlphaLISA SureFire Ultra, Alpha SureFire Ultra Multiplex and LANCE Ultra cAMP assays
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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