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Extra control lysate for the AlphaLISA® SureFire® Ultra™ Human Phospho-ß-Catenin (Ser37) assay. This control is already provided in the AlphaLISA SureFire Ultra Human Phospho-ß-Catenin (Ser37) assay kit, but can also be ordered separately.
This control lysate is intended for use with the AlphaLISA SureFire Ultra Human Phospho-ß-Catenin (Ser37) Detection Kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, A549 cells were cultured to confluence in T175 flasks in 10% FBS containing medium. Cells were treated with 1 μg/mL PMA and 50 nM Calyculin A for 4 hours at 37°C. Cells were lysed in 5 mL of 1X SureFire Ultra Lysis Buffer.
AlphaLISA SureFire Ultra
Central Nervous System