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AlphaLISA SureFire Ultra Human Total c-Met Detection Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra Human Total c-Met assay is a sandwich immunoassay for quantitative detection of total c-Met in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Part number: ALSU-TCMET-A500
Unit Size: 500 Assay Points
List price: USD 2,346.00
Your price:
USD 2,346.00
USD 2,346.00 /each
Part number: ALSU-TCMET-A10K
Unit Size: 10,000 Assay Points
List price: USD 14,270.00
Your price:
USD 0.00
USD 14,270.00 /each
Part number: ALSU-TCMET-A50K
Unit Size: 50,000 Assay Points
List price: USD 46,060.00
Your price:
USD 0.00
USD 46,060.00 /each
Part number: ALSU-TCMET-A-HV
Unit Size: 100 Assay Points
List price: USD 694.44
Your price:
USD 0.00
USD 694.44 /each

Overview

C-Met, also known as tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGFR) is encoded by a proto-oncogene and is created as a single-chain precursor before being converted to its mature form. The hepatocyte growth factor (HGF) can activate c-Met, a receptor tyrosine kinase that upon HGF binding undergoes dimerization and autophosphorylation (Y1234 and Y1235 residues) stimulating several downstream signaling pathways involved in cell proliferation, angiogenesis, and tumor growth. In cancer cells, abnormal MET downstream signaling is closely related to overexpression and amplification. c-Met is overexpressed in up to 80% of renal cell carcinoma (RCC) and 25-75% of non-small cell lung cancer (NSCLC).

The AlphaLISA SureFire Human Total c-Met Detection Kit is a sandwich immunoassay for the quantitative detection of total c-Met in cellular lysates, using Alpha Technology.

Formats
  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
AlphaLISA SureFire Ultra kits are compatible with
  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies
Alpha SureFire kits can be used for
  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Specifications

Application
Protein Analysis & Detection
Protein Detection
Assay Target Class
Phospho-protein
Assay Technology
Alpha
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra
Detection Method
Alpha
Experimental Type
In vitro
Target Species
Human
Therapeutic Area
Oncology
Unit Size
500 Assay Points

Video gallery

Resources

1-3 of 3 Resources
Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

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Brochure
Alpha SureFire Ultra no-wash immunoassay catalog

Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...

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Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra

Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...

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SDS, COAs, Manuals and more

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