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AlphaLISA SureFire Ultra Human and Mouse Phospho-c-Jun (Ser73) Detection Kit, 100 Assay Points

The AlphaLISA® SureFire® Ultra Human and Mouse Phospho-c-Jun (Ser73) assay is a sandwich immunoassay for quantitative detection of phospho-c-Jun (Ser73) in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-PCJUN-B500
Unit Size:
Part Number: ALSU-PCJUN-B10K
Unit Size:
Part Number: ALSU-PCJUN-B50K
Unit Size:
Part Number: ALSU-PCJUN-B-HV
Unit Size:
  • c-Jun belongs to the mammalian Jun protein family which are transcription factors that must dimerize to bind to their target DNA sites. c-Jun is a major component of the activator protein 1 (AP-1) complex. Phosphorylation of c-Jun at Ser63 and Ser73 through JNK stimulates AP-1. Once phosphorylated, c-Jun can dimerize and bind to DNA sequences and affect transcription. c-Jun regulates numerous cellular responses including proliferation, malignant transformation, differentiation, and apoptosis. C-Jun plays a role in many types of cancers and autoimmune diseases.

    The AlphaLISA SureFire Human and Mouse Phospho-c-Jun (Ser73) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-c-Jun (Ser73) in cellular lysates, using Alpha Technology.

    Formats
    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
    AlphaLISA SureFire Ultra kits are compatible with
    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies
    Alpha SureFire kits can be used for
    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Application
    Protein Analysis & Detection
    Protein Detection
    Assay Points
    100
    Assay Target Class
    Phospho-protein
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Target Species
    Human
    Mouse
    Therapeutic Area
    Autoimmunity
    Oncology

Resources

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Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra
surefire, neutralizing antibody, neutralizing antibodies, biotherapeutics, biotx