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AlphaLISA SureFire Ultra Human and Mouse Total BRD9 Detection Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra Human and Mouse Total BRD9 assay is a sandwich immunoassay for quantitative detection of total BRD9 in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-TBRD9-A500
Unit Size: 500 Assay Points
Part Number: ALSU-TBRD9-A10K
Unit Size: 10,000 Assay Points
Part Number: ALSU-TBRD9-A50K
Unit Size: 50,000 Assay Points
Part Number: ALSU-TBRD9-A-HV
Unit Size: 100 Assay Points
  • BRD9 (Bromodomain-containing protein 9) is closely related to Bromodomain-containing protein 7 (BRD7), both of which are members of the Bromodomain-containing Proteins Family IV. BRD9 binds acetylated lysine residues of histones, mediating chromatin remodeling and regulating transcription and is therefore involved in pluripotency regulation and cell development. BRD9 overexpression is associated with neurodevelopmental disorders and cancers, such as acute myeloid leukemia. Targeting BRD9 degradation with PROTAC molecules has emerged as a novel therapeutic approach. Since BRD9 and BRD7 proteins share 72% residue similarity, compounds are likely to target both the pro-oncogene BRD9 and the tumor suppressor BRD7, suggesting that selectivity of compounds targeting either BRD9 or BRD7 must be carefully investigated.

    The AlphaLISA SureFire Human and Mouse Total BRD9 Detection Kit is a sandwich immunoassay for the quantitative detection of total BRD9 in cellular lysates, using Alpha Technology.

    Formats
    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
    AlphaLISA SureFire Ultra kits are compatible with
    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies
    Alpha SureFire kits can be used for
    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Application
    Protein Analysis & Detection
    Protein Detection
    Assay Points
    10000
    Assay Target Class
    Phospho-protein
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Shipping Conditions
    Shipped in Blue Ice
    Target Species
    Human
    Mouse
    Therapeutic Area
    Oncology
    Unit Size
    10,000 Assay Points

Resources

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Brochure
Alpha SureFire Ultra no-wash immunoassay catalog

Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...

Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

Application Note Icon Image
Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra

Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...