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AlphaLISA SureFire Ultra Human Total BRD4 Detection Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra Human Total BRD4 assay is a sandwich immunoassay for quantitative detection of total BRD4 in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: ALSU-TBRD4-A500
Unit Size: 500 Assay Points
List price: USD 1,849.00
Your price:
USD 0.00
USD 1,849.00 /each
Part number: ALSU-TBRD4-A10K
Unit Size: 10,000 Assay Points
List price: USD 11,440.00
Your price:
USD 0.00
USD 11,440.00 /each
Part number: ALSU-TBRD4-A50K
Unit Size: 50,000 Assay Points
List price: USD 32,520.00
Your price:
USD 32,520.00
USD 32,520.00 /each
Part number: ALSU-TBRD4-A-HV
Unit Size: 100 Assay Points
List price: USD 639.89
Your price:
USD 0.00
USD 639.89 /each

Overview

Bromodomain-containing protein 4 (BRD4) is a member of the Bromodomains and Extraterminal (BET) family, consisting of BRD2, BRD3, and BRDT. BRD4 is a transcription regulator consisting of two bromodomains which bind acetylated lysine residues on proteins, such as histones. In association with Cyclin T1 and CDK9, BRD4 forms a complex called P-TEFb which controls gene transcription. BRD4 is also involved in DNA damage response, embryogenesis, and cancer development. Inhibiting BRD4 is a strategy for treating cancer, and more recently targeting BRD4 with PROTAC molecules has emerged as a novel therapeutic approach.

The AlphaLISA SureFire Human Total BRD4 Detection Kit is a sandwich immunoassay for the quantitative detection of total BRD4 in cellular lysates, using Alpha Technology.

Formats
  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
AlphaLISA SureFire Ultra kits are compatible with
  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies
Alpha SureFire kits can be used for
  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Specifications

Application
Protein Analysis & Detection
Protein Detection
Assay Points
50000
Assay Target Class
Phospho-protein
Assay Technology
Alpha
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra
Detection Method
Alpha
Experimental Type
In vitro
Shipping Conditions
Shipped in Blue Ice
Target Species
Human
Therapeutic Area
Oncology
Unit Size
50,000 Assay Points

Video gallery

Resources

1-3 of 3 Resources
Guide Icon
Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

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Brochure
Alpha SureFire Ultra no-wash immunoassay catalog

Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...

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Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra

Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...

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SDS, COAs, Manuals and more

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