Bromodomain contain 3 protein (BRD3) is a member of the Family II of Bromodomains, which includes BRD2 and BRD4. BRD3 associates with transcription complexes and acetylated chromatin during mitosis and plays an important role in gene transcription. BRD2 and BRD3 perform overlapping cellular functions which suggests that the simultaneous disruption or depletion of these proteins may represent a promising drug therapeutic strategy against cancer. Dysregulation of BRD3 is associated with a variety of cancers including breast, prostate, and medulloblastoma.

The AlphaLISA SureFire Human Total BRD3 Detection Kit is a sandwich immunoassay for the quantitative detection of total BRD3 in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening