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AlphaLISA SureFire Ultra Human Total BCL-2 Detection Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra Human Total BCL-2 assay is a sandwich immunoassay for quantitative detection of total BCL-2 in cellular lysates using Alpha Technology.

Product Variants
Part number: ALSU-TBCL2-A500
Unit Size: 500 Assay Points
List price: USD 2,346.00
Your price:
USD 2,346.00
USD 2,346.00 /each
Part number: ALSU-TBCL2-A10K
Unit Size: 10,000 Assay Points
List price: USD 14,270.00
Your price:
USD 0.00
USD 14,270.00 /each
Part number: ALSU-TBCL2-A50K
Unit Size: 50,000 Assay Points
List price: USD 46,060.00
Your price:
USD 0.00
USD 46,060.00 /each
Part number: ALSU-TBCL2-A-HV
Unit Size: 100 Assay Points
List price: USD 694.44
Your price:
USD 0.00
USD 694.44 /each

Overview

The oncogenic protein BCL-2 (B-cell lymphoma 2) is known for its role in preventing apoptosis. It is a 24-26 kDa protein that is found in the nuclear envelope, endoplasmic reticulum, and mitochondrial membrane. BCL-2 is categorized as a proto-oncogene since it is thought to be a significant antiapoptotic protein. Numerous cancers, such as breast, prostate, skin, colon, and pancreatic cancers, as well as neurological and autoimmune illnesses, have been linked to BCL-2 overexpression. The anti-apoptotic proteins BCL-XL, MCL-1, and BCL-W are all related. The BCL-2 family of proteins contains proapoptotic proteins such as Bax, BAD, Bak, Blk, and Bid.

The AlphaLISA SureFire Human Total BCL-2 Detection Kit is a sandwich immunoassay for the quantitative detection of total BCL-2 in cellular lysates, using Alpha Technology.

Formats:
  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
AlphaLISA SureFire Ultra kits are compatible with:
  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies
Alpha SureFire kits can be used for:
  • Cellular kinase assays
  • Receptor activation studies
  • Screening

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Specifications

Application
Protein Analysis & Detection
Protein Detection
Assay Target Class
Phospho-protein
Assay Technology
Alpha
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra
Detection Method
Alpha
Experimental Type
In vitro
Shipping Conditions
Shipped in Blue Ice
Target Species
Human
Therapeutic Area
Oncology
Unit Size
500 Assay Points

Video gallery

Resources

1-2 of 2 Resources
Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

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Alpha SureFire Ultra no-wash immunoassay catalog

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