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AlphaLISA SureFire Ultra Human Phospho-BCL-2 (Ser70) Detection Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra Human Phospho-BCL-2 (Ser70) assay is a sandwich immunoassay for quantitative detection of phospho-BCL-2 (Ser70) in cellular lysates using Alpha Technology.

Part Number: ALSU-PBCL2-A500
Unit Size:
Part Number: ALSU-PBCL2-A10K
Unit Size:
Part Number: ALSU-PBCL2-A50K
Unit Size:
Part Number: ALSU-PBCL2-A-HV
Unit Size:
  • The oncogenic protein BCL-2 (B-cell lymphoma 2) is known for its role in preventing apoptosis. It is a 24-26 kDa protein that is found in the nuclear envelope, endoplasmic reticulum, and mitochondrial membrane. BCL-2 is categorized as a proto-oncogene since it is thought to be a significant antiapoptotic protein. Numerous cancers, such as breast, prostate, skin, colon, and pancreatic cancers, as well as neurological and autoimmune illnesses, have been linked to BCL-2 overexpression. The anti-apoptotic proteins BCL-XL, MCL-1, and BCL-W are all related. The BCL-2 family of proteins contains proapoptotic proteins such as Bax, BAD, Bak, Blk, and Bid.

    The AlphaLISA SureFire Human Phospho-BCL-2 (Ser70) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-BCL-2 (Ser70) in cellular lysates, using Alpha Technology.

    Formats:
    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
    AlphaLISA SureFire Ultra kits are compatible with:
    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies
    Alpha SureFire kits can be used for:
    • Cellular kinase assays
    • Receptor activation studies
    • Screening

    For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

  • Application
    Protein Analysis & Detection
    Protein Detection
    Assay Target Class
    Phospho-protein
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Target Species
    Human
    Therapeutic Area
    Oncology