ATG14, or autophagy related protein 14, also known as BAKOR, or Beclin 1-associated autophagy-related key regulator, is an important component of the autophagosome nucleation process in macroautophagy. Dysfunctions in autophagic mechanisms are associated with cancer and neurodegenerative disease. ATG14 is implicated in Alzheimer’s disease, and a variety of cancers such as colorectal cancer, breast cancer, and chronic myeloid leukemia.

The AlphaLISA SureFire Ultra Human and Mouse Phospho-ATG14 (Ser29) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-ATG14 (Ser29) in cellular lysates, using Alpha Technology.

 Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• Screening