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AlphaLISA SureFire Ultra Human and Mouse Total ATF-4 Detection Kit, 100 Assay Points

The AlphaLISA® SureFire® Ultra Human and Mouse Total ATF-4 assay is a sandwich immunoassay for quantitative detection of total ATF-4 in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-TATF4-A500
Unit Size: 500 Assay Points
Part Number: ALSU-TATF4-A10K
Unit Size: 10,000 Assay Points
Part Number: ALSU-TATF4-A50K
Unit Size: 50,000 Assay Points
Part Number: ALSU-TATF4-A-HV
Unit Size: 100 Assay Points
  • Activating transcription factor 4 (ATF-4), also known as tax-responsive enhancer element B67, is a multifunctional transcription regulatory protein in the basic leucine zipper superfamily. ATF-4 is a member of a family of DNA-binding proteins that includes AP-1 family, CREBs, and CREB-like proteins, all of which share a leucine zipper region that is involved in protein-protein interactions. Under stress conditions, PERK pathway rapidly stimulates the expression of NRF2 through activating transcription factor 4 (ATF4) that contributes to the NRF2 protective response.

    The AlphaLISA SureFire Human and Mouse Total ATF-4 Detection Kit is a sandwich immunoassay for the quantitative detection of total ATF-4 in cellular lysates, using Alpha Technology.

    Formats
    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
    AlphaLISA SureFire Ultra kits are compatible with
    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies
    Alpha SureFire kits can be used for
    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Application
    Protein Analysis & Detection
    Protein Detection
    Assay Target Class
    Phospho-protein
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Target Species
    Human
    Mouse
    Therapeutic Area
    Oncology
    Unit Size
    100 Assay Points

Resources

1-4 of 4 Resources
Brochure Icon Image
Brochure
Alpha SureFire Ultra no-wash immunoassay catalog

Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...

Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

Application Note Icon Image
Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra

Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...

Technical Note
Evaluating protein phosphorylation and cellular signaling cascades within the integrated stress response pathway

Protein phosphorylation, regulated by kinase and phosphatase cascades, plays a crucial role in cellular processes. Monitoring...