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AlphaLISA Surefire Ultra Ultra Human, Mouse, and Rat Phospho-AKT1 (Ser473) Detection Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ phospho-AKT1 (Ser473) assay kit can be used to measure levels of AKT1 phosphorylated at residue Ser473 in cellular lysates using no-wash Alpha technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-PAKT1-C500
Unit Size: 500 Assay Points
Part Number: ALSU-PAKT1-C10K
Unit Size: 10,000 Assay Points
Part Number: ALSU-PAKT1-C50K
Unit Size: 50,000 Assay Points
Part Number: ALSU-PAKT1-C-HV
Unit Size: 100 Assay Points
  • Formats:

    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 µL reaction volume.

    In the AlphaLISA®SureFire®Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.

    AlphaLISA®SureFire®Ultra™ kits are compatible with:

    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies

    Alpha SureFire® kits can be used for:

    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Application
    Kinase
    Protein Analysis & Detection
    Assay Pathway
    PI3K/Akt/mTOR
    Assay Points
    50000
    Assay Target Class
    Phospho-protein
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Molecular Modification
    Phosphorylation
    Therapeutic Area
    Cardiovascular
    Central Nervous System
    Metabolic
    Unit Size
    50,000 Assay Points

Resources

1-4 of 4 Resources
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Brochure
Alpha SureFire Ultra no-wash immunoassay catalog

Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...

Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

Application Note Icon Image
Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra

Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...

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