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The AlphaLISA® SureFire® Ultra™ total Akt1/2/3 assay is a sandwich immunoassay for quantitative detection of Akt1/2/3 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization or control assay in conjunction with the AlphaLISA SureFire phospho-Akt1/2/3 assay.
This kit has been pre-validated for use in combination with Pelago Bioscience’s CETSA® (Cellular Thermal Shift Assay) technology. Please note that further assay optimization will be required in order to adapt the kit to your particular cell line and experimental conditions. A valid CETSA® licence from Pelago Bioscience or a single kit agreement is required for using the CETSA® technology.
In the AlphaLISA SureFire Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.
AlphaLISA SureFire Ultra™ kits are compatible with:
Alpha SureFire kits can be used for:
Protein Analysis & Detection
|Assay Target Class
AlphaLISA SureFire Ultra
Central Nervous System