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AlphaLISA Surefire Ultra Human, Mouse, and Rat Phospho-AKT 1/2/3 (pS473) Detection Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra p-AKT1/2/3 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-AKT1/2/3 (phosphorylated on Ser473) in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: ALSU-PAKT-B500
Unit Size: 500 assay points
List price: USD 2,346.00
Your price:
USD 2,346.00
USD 2,346.00 /each
Part number: ALSU-PAKT-B10K
Unit Size: 10,000 Assay Points
List price: USD 14,270.00
Your price:
USD 0.00
USD 14,270.00 /each
Part number: ALSU-PAKT-B50K
Unit Size: 50,000 assay points
List price: USD 44,270.00
Your price:
USD 0.00
USD 44,270.00 /each
Part number: ALSU-PAKT-B-HV
Unit Size: 100 assay points
List price: USD 694.44
Your price:
USD 0.00
USD 694.44 /each



  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 µL reaction volume.

In the AlphaLISA®SureFire®Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.

AlphaLISA®SureFire®Ultra kits are compatible with:

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening


Assay Pathway
Assay Target Class
Automation Compatible
AlphaLISA SureFire Ultra
Detection Method
One Unit Contains
1.0 each
Quantity in a Package Amount
500.0 Units
Therapeutic Area
Central Nervous System
Unit Size
500 assay points

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1-6 of 7 Resources
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AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

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Alpha SureFire Ultra no-wash immunoassay catalog

Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...

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An overview of atherosclerosis

Atherosclerosis pathogenesis, cellular actors, and pathways

Atherosclerosis is a common condition in which arteries harden and...

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Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra

Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...

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Application Note
Characterizing chemokine receptor inhibitors with AlphaLISA SureFire Ultra, Alpha SureFire Ultra Multiplex and LANCE Ultra cAMP assays

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...

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Protein degradation awareness in a single guide

An in-depth review of molecular and cellular pathways

The maintenance of proteostasis, the biological mechanisms that control the...

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SDS, COAs, Manuals and more

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