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AlphaLISA Surefire Ultra Human, Mouse, and Rat Phospho-4E-BP1 (Thr37/46) Detection Kit, 100 Assay Points

The AlphaLISA® SureFire® Ultra™ p-4E-BP1 (Thr37/46) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr37/46) in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-P4EBP-A500
Unit Size: 500 assay points
Part Number: ALSU-P4EBP-A10K
Unit Size: 10,000 Assay Points
Part Number: ALSU-P4EBP-A50K
Unit Size: 50,000 assay points
Part Number: ALSU-P4EBP-A-HV
Unit Size: 100 assay points
  • The AlphaLISA®SureFire®Ultra p-4E-BP1 (Thr37/46) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr37/46) in cellular lysates using Alpha Technology.

    In the AlphaLISA®SureFire®Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal.  The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.

    AlphaLISA®SureFire®Ultra kits are compatible with:

    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies

    Alpha SureFire® kits can be used for:

    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Assay Pathway
    PI3K/Akt/mTOR
    Assay Points
    100
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Format
    Microplates
    Quantity in a Package Amount
    1.0 per pack
    Therapeutic Area
    Metabolic
    Unit Size
    100 assay points

Resources

1-2 of 2 Resources
Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

Application Note Icon Image
Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra

Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...