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AlphaScreen Anti-GFP Donor Beads, 5 mg

Alpha Donor beads conjugated to anti-GFP antibody. The antibody is a mouse monoclonal antibody that recognizes wildtype and recombinant GFP (rGFP), and cross-reacts with eGFP. These beads can be used to capture GFP-tagged proteins for AlphaScreen, AlphaLISA, and AlphaPlex no-wash assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AS112D
Unit Size:
Part Number: AS112M
Unit Size:
Part Number: AS112R
Unit Size:
  • These beads can be used in conjunction with AlphaScreen, AlphaLISA, or AlphaPlex beads to create Alpha no-wash immunoassays for:

    • Protein-protein interaction assays
    • Protein detection assays
    • Enzymatic assays
    • Other immunoassays

    In a typical Alpha assay, 1 mg of Donor beads is sufficient to run 1,000-2,000 wells using a 25 µL reaction volume.

    Features:

    • No-wash steps, no separation steps
    • Ease-of-use: few addition steps, fast assay development
    • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
    • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
    • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders

    AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym "Alpha" stands for amplified luminescent proximity homogeneous assay. As the name implies, some of the key features of these technologies are that they are non-radioactive, homogeneous proximity assays. Binding of molecules captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent/fluorescent signal. To understand how a signal is produced, one must begin with an understanding of the beads. AlphaScreen and AlphaLISA assays require two bead types: Donor beads and Acceptor beads. Each bead type contains a different proprietary mixture of chemicals, which are key elements of the AlphaScreen technology. Donor beads contain a photosensitizer, phthalocyanine, which converts ambient oxygen to an excited and reactive form of O2, singlet oxygen, upon illumination at 680 nm. Please note that singlet oxygen is not a radical; it is molecular oxygen with a single excited electron. Like other excited molecules, singlet oxygen has a limited lifetime prior to falling back to ground state. Within its 4 µsec half-life, singlet oxygen can diffuse approximately 200 nm in solution. If an Acceptor bead is within that proximity, energy is transferred from the singlet oxygen to thioxene derivatives within the Acceptor bead, subsequently culminating in light production at 520-620 nm (AlphaScreen) or at 615 nm (AlphaLISA). In the absence of an Acceptor bead, singlet oxygen falls to ground state and no signal is produced. This proximity-dependent chemical energy transfer is the basis for AlphaScreen's homogeneous nature.

  • Antibody Conjugates
    Anti-GFP
    Application
    Protein-protein interactions
    Protein Analysis & Detection
    Automation Compatible
    Yes
    Bead Type or Core Bead Type
    Alpha Donor
    Brand
    AlphaLISA
    Detection Method
    Alpha
    Format
    Microplates

Resources

1-3 of 3 Resources
Application Note Icon Image
Application Note
A Fast and Simple Chemiluminescent Assay for Monitoring of DNA-Protein Interactions
dna-protein, dna:protein, binding, interaction, protein:dna, protein-dna, alphalisa, emsa
Guide
Alpha Protein-Protein Interaction Quick Start Guide
alphalisa, protein, assay, immunoassay
Guide
ELISA to AlphaLISA Immunoassay Conversion Guide
elisa conversion, alphalisa, long guide