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ATPlite 1glow-F, 450 ml

ATPlite™ 1glow F is an advanced luminescence assay that detects ATP in 2D and 3D mammalian cell cultures using firefly luciferase. This assay is available in a ready-to-use frozen liquid format for high-throughput applications. ATPlite 1glow F is designed for enhanced signal stability and sensitivity compared to traditional colorimetric and fluorometric viability methods.

Feature Specification
Application Cell Viability
Assay Points 4,500 pts, 96-well: 18,000 pts, 384-well

ATPlite™ 1glow F is an advanced luminescence assay that detects ATP in 2D and 3D mammalian cell cultures using firefly luciferase. This assay is available in a ready-to-use frozen liquid format for high-throughput applications. ATPlite 1glow F is designed for enhanced signal stability and sensitivity compared to traditional colorimetric and fluorometric viability methods.

Product variants
Unit Size: 10 mL
Part #:
6096736
List price
USD 72.00
Your online price:
Unit Size: 100 mL
Part #:
6096731
List price
USD 400.00
Your online price:
Unit Size: 450 mL
Part #:
6096739
List price
USD 1,700.00
Your online price:
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).

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Overview

ATP is a marker for cell viability due to its presence in all metabolically active cells. ATP concentration declines rapidly when cells undergo necrosis or apoptosis, and so monitoring ATP is a good indicator of cytotoxic, cytostatic and proliferation effects.

Our ATPlite 1glow F, in a ready-to-use liquid format, is an advanced luminescence assay that detects ATP in mammalian cells using firefly luciferase. As ATP levels correlate with cell viability, this assay is a powerful tool for assessing cytotoxicity and proliferation. Engineered for signal stability, this luminescence based technology provides a broad dynamic range and can reduce interference from colored compounds that may affect absorbance-based viability assays.

The ATPlite 1glow F reagent utilizes a homogenous "add-mix-read" format, eliminating wash and separation steps to simplify workflows in high-throughput applications. It features enhanced signal stability with a long signal half-life, offering greater flexibility for batch processing. This single reagent measures ATP changes in both 2D and 3D cell culture.

Specifications

Application
Cell Viability
Assay Points
4,500 pts, 96-well: 18,000 pts, 384-well
Automation Compatible
Yes
Brand
ATPlite 1glow
Cellular or Signaling Pathway
Autophagy
Cell Death
Cell Growth
Cytotoxicity
Necrosis
Detection Modality
Luminescence
Format
Microplates
Protocol
ready-to-use
Signal Halflife
> 3 hours
Shipping Conditions
Shipped in Dry Ice
Unit Size
450 mL

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